inner1

Clinical Testing

Sr.No. Specimen Collection / Transportation
1. Urine Culture Sterile Container
2. Pus Culture Sterile Container / Transport Swab
3 Sputum culture Sterile Container
4. Sputum AFB Culture or Mycobacteria culture Sterile Container
5. Eye Discharge Sterile Container
6. Ear Discharge Sterile Container
7. CSF Culture Sterile Container
8. CSF Culture (Pyogenic by BACTEC – 9050) Sterile Container
9. CSF Culture (Mycobacteria,Fungus,Yeast by BACTEC-9050) Sterile Container
10. CSF (cryptococcus Antigen) Sterile Container
11. Pyogenic culture and TB culture for any body fluid including Pleural fluid, Pericardial fluid, etc. Bactec Media / Sterile SPS Container
12. Blood Culture – (Aerobic organisms) Bactec Media
13. Blood Culture – (Aerobic organisms) Bactec Media
14. Swab Culture – (Pyogenic organisms) Bactec Media
15. Semen Culture Sterile Container
16. AFB Culture (Any Specimen) on L.J. medium Sterile Container
17. AFB Culture (Any Specimen) by BACTEC Sterile Container
18. AFB Culture (Blood, body fluid) by BACTEC Sterile Container
19. Fungus Culture Sterile Container
20. Fungus sensitivity by ATB Fungus (Yeast isolates only) Sterile Container
21. MTD Test for direct detection of Mycobacterium tuberculosis Sterile Container
22. Bacterial Antigen Sterile Container
23. Aspergillosis Serology Sterile Container
24. PACE – 2 Chlamydia & gonorrhea Sterile Container
25. TPHA Sterile Container
26. Cryptococcus Antigen Detection Sterile Container
27. Candida Antigen Detection Sterile Container
28. ASO titre Sterile Container
29. RA Sterile Container
30. RPR Sterile Container
31. Blood Grouping Sterile Container
32. Widal test Sterile Container
33. P.falciparum antigen detection Sterile Container
34. Antibodies (IgG) for H. pylori Sterile Container
35. Gastric biopsy for H. pylori Sterile Container
36. HIV 1+2 (Spot) Sterile Container
37. HBsAg Sterile Container
38. Pregnancy Test Sterile Container

Detailed Menu
 
Urine Culture
Collection : As per one of the following standard methods MSU / Catheter / Suprapubic, after pro static massage and before pro static massage.
Transportation : Deep slide / Container with preservative
Reporting : Within 24 to 48 hours
Antibiogram : See Annexure :1
     
bullet
Pus Culture
Collection : Collect in a sterile container
Transportation : Swab in transport media supplied
Reporting : Within 24 to 48 hours
Antibiogram : See Annexure :1
Additional : Two smears on glass slide should be sent with the specimen
 
bullet
Sputum Culture
Collection :

Transportation : Send transport swab along with well prepared air dried and fixed smears on glass slide &remaining sputum specimen.
     
bullet
Sputum AFB Culture / Mycobacteria Culture
Method -1 : L J
Reporting : Preliminary: 3 weeks and Final report : 8 weeks
Method -2 : BACTEC MGIT (Florescent)
Collection : As stated above however, atleast 3 specimen should be collected In case of smear negative cases and 5 specimen in suspected doubtful cases.

Reporting : Preliminary : 7 days and Final report : 45 days
Antibiogram : See Annexure : 2
     
bullet
Eye Discharge
Collection : Use sterile swab to collect discharge and then insert it in transport media.
Transportation : Use Amies transport media
Reporting : 48 hours
Antibiogram : See Annexure :1
     
bullet
Ear Discharge
Collection : media :

(1) Insert swab after aseptically collecting from discharging ear Into Amies transport.

(2) Make two smears on a clean glass slide, air dry it and fix it.


Transportation : Use Amies transport media
Reporting : 48 hours
Antibiogram : See Annexure :1
     
   
bullet
CSF Culture
Collection : Collect in a sterile screw cap tube or sterile vaccutainer only.
Method : Routine
Reporting : 48 to 72 hours
Antibiogram : See Annexure :1
 
bullet
CSF Culture (Pyogenic by BACTEC – 9050)
Collection : Collect in a sterile screw cap tube or sterile vaccutainer only. Sterile Heparin / EDTA
Method : BACTEC-9050 using aerobic and anaerobic resin (Bactec ) media. vaccutainer
Reporting : 24 to 48 hours
Antibiogram : See Annexure :1
     
bullet
CSF Culture (Mycobacteria, Fungus ,Yeast by BACTEC – 9050)
Collection : Collect in a sterile screw cap tube or sterile vaccutainer only.
Method : BACTEC – 9050 Using Myco / Lytic Media
Reporting : Preliminary report : 7 days & Final report : 45 days
Antibiogram : See Annexure :3
     
bullet
CSF (Cryptococcus Antigen)
Collection : CSF in a sterile container
Method : Latex Agglutination
Reporting : The same day
     
bullet
Pyogenic Culture & TB Culture
Collection : Collect in a sterile screw cap tube or sterile vaccutainer only.
Specimen : Any Body Fluid including Pleural Fluid,Pericardial fluid,synovial Fluid etc.
Method : BACTEC-9050 using aerobic/Anaerobic resin Media (bactec).
Reporting : Within 24 to 48 hours
Antibiogram : See Annexure :1
 
bullet
Blood Culture (Aerobic organisms)
Collection : 5 ml blood in case of adult & 1 ml in Paediatric vial, before Giving any antibiotic.
Method : Manual
Reporting : Preliminary report after 48 hours & Final report – 7 days
Antibiogram : See Annexure :1
     
bullet
Blood Culture (Aerobic organisms)
Collection : 5 ml blood in Aerobic+ in case of adult & 1 ml for Peds + Plus vials.
Transportation : Incubate it at 35 degree C Storage before
Reporting : Preliminary report withi 48 hours & Final report – 7 days
Method : BACTEC 9050 (automatic)
Antibiogram : See Annexure :1
 
bullet
Swab Culture (Pyogenic organisms)
Collection : Collect cotton swab supplied along with transport media by Aseptic technique, insert swab in a Amies/Stuart transport Media.